Robea Ballo, Dong-Ping Li, M. Iqbal Parker
Genotyping of Alcohol Dehydrogenase Type 2
and 3 Using a Two-Buffer Polyacrylamide Gel
Electrophoresis System
Genetic polymorphisms in the alcohol dehydrogenase
genes, ADH2 and ADH3, have been shown to affect individual
susceptibility to diseases such as alcoholism and
oesophageal cancer. Although several PCR-based
methods for genotyping these enzymes have been previously
developed, the two-buffer polyacrylamide gel
electrophoresis system has the ability to rapidly resolve
all classes of point mutations within 2–3 hours instead
of the conventional overnight separation. The success
of this technique is partly attributable to a discontinuous
two-phase buffer system and horizontal flatbed gel
electrophoresis rather than conventional vertical gels.
Using a modification of this system, we were able to detect
all of the known polymorphisms within ADH2 exons
3 and 9 and ADH3 exon 8, as well as a rare variant
within ADH2 exon 9. This method is rapid, cost-effective,
and is ideal for large scale screening programmes.
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 41, 03/2003
Pages: 298 - 301
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