Luigia Iamele, Roberto Fiocchi, Arialdo Vernocchi
Evaluation of an Automated Spectrophotometric Assay for Reactive Oxygen Metabolites in Serum
The in vivo assessment of free radicals concentration
is hampered by their instability and extremely short
half-life. The Diacron Reactive Oxygen Metabolites
(D-ROM) test is a recently introduced method to evaluate
the peroxidation of organic compounds. Since the
manual performance of the test provides excessive analytical
imprecision, the aim of this study was to evaluate
the automation of this test. Within- and between-run
imprecision and interference were assessed
according to the guidelines proposed by the NCCLS.
The reactive oxygen metabolites' (ROM) stability was
evaluated in different physical conditions. For withinrun
and between-run imprecision the coefficients of
variation were consistently lower than 5%. The maximum
allowable concentration was 28.2 mmol/l,
0.068 mmol/l and 171 mmol/l for triglycerides, haemoglobin
and bilirubin, respectively. Serum storage at ?20°C provided adequate ROM stability for up to 3
months, whereas storage at 4°C yielded non-reproducible
results. In conclusion, our data provide evidence
that the D-ROM assay has both an acceptable
stability and an adequate imprecision. The automated
assay may be regarded as a fast and reproducible
method for the quantitative evaluation of oxidative
stress. Since it is easily performed, the method is suitable
for routine in clinical laboratories and may provide
an accurate estimation of oxidative stress in vivo.
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 40, 07/2002
Pages: 673 - 676
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