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Giuseppe Lippi, Orazio Ruzzenente, Claudio Brentegani, Anna Pierotti, Giancesare Guidi
Evaluation of the Analytical Performances of a New Fully Automated Commercial Immunonephelometric Assay for Lipoprotein(a)
Several commercial methods have been proposed for lipoprotein(a) (Lp(a)) measurements over the past decades. However, only a few of them appear completely suitable in terms of analytical performance, costs and practicability. We evaluated the analytical performance of a new commercial fully automated immunonephelometric assay for Lp(a) measurements on the IMMAGE® Immunochemistry System. Mean within- and between-run coefficients of variation were 2.7 % (range 1.2–4.7 %) and 3.8 % (range 1.8–7.9 %), respectively. The linearity of the assay was confirmed up to 102 mg/dl and the deviation from the expected values did not exceed 4% (mean deviation: 1.9%). Moreover, the relative non-linearity was acceptable, ranging from 1.4 % to 1.6 % and hence constantly lower than the 2.5 % upper limit. Since there is no reference method for Lp(a) measurements, 100 routine random serum samples measured by the IMMAGE® Immunochemistry System immunonephelometric assay were further compared with two other commercial immunonephelometric assays (Array® LPA immunonephelometric assay and BNA Latex-Enhanced Lp(a) nephelometric assay). Non-parametric regression and relative Spearman's correlation coefficients were satisfactory, (
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 36, 09/1998
Pages: 719 - 723