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Burcin zyaman, Axel Gdecke, Susanne Ksters, Elisabeth Kirchhoff, Rdiger E. Scharf, Jrgen Schrader
Endothelial nitric oxide synthase plays a minor role in inhibition of arterial thrombus formation
Endothelial NO synthase (eNOS) expressed in the vascular endothelium or formed within platelets was postulated to inhibit platelet activation and aggregation. We have assessed the role of eNOS in platelet aggregation in vitro and in vivo by comparison of WT and eNOS-/- mice. Aggregometer studies revealed that collagen over a concentration range of 0.3610 g aggregated WT and eNOS-/- platelets to the same extent (10 g: WT 86.74.7%, eNOS-/- 9112%, n=6). Collagen treatment did not result in a significant increase in cGMP formation and VASP phosphorylation. Thrombin-induced P-selectin surface expression was unchanged in eNOS-/-platelets. In line with these findings no eNOS protein was detectable within the platelets of WT mice. In vivo, bleeding time after tail tip resection tended to be shorter in eNOS/- mice (WT: 11635 s; eNOS-/- 10937 s, n.s). Similarly, time to occlusion of the A.carotis after focal induction of thrombosis was 50176 s (WT) and 45795 s (eNOS-/-) (n.s.). These data demonstrate that eNOS-deficiency minimally affects platelet aggregation and is not associated with accelerated arterial thrombosis in vivo. Thus, in the mouse endothelial NO synthase does not play a major role in the autocrine modulation of platelet function and in thrombosis of conduit vessels in vivo.
Thrombosis and Haemostasis, Schattauer
Print ISSN: 0340-6245
Volume: 93, 06/2005
Pages: 1161 - 1167