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Anne-Marie Fahr, Ulrich Eigner, Pramod M. Shah

Einfluss der verzögerten Transportdauer bei Raumtemperatur auf die Rate der falsch negativen Blutkulturen im BACTEC? 9000 Gerät. Impact of delayed entry at room temperature on the rate of false negative blood cultures with the BACTEC? 9000 instrument

Keywords: BACTEC? 9000, Blutkulturdiagnostik, sepsis

One hospital-based laboratory and one laboratory serving a number of hospitals prospectively studied 3.907 blood culture bottles of the BACTEC? 9000 System (BD Diagnostics, Heidelberg, Germany) (1.888 aerobic bottles, 1.880 anaerobic bottles and 139 pediatric bottles). Information on media type, blood volume, time of inoculation, entry into the system, anti-microbial treatment, time to detection, identification to the species level and positive rate compared to terminal subculture were recorded and analysed.

Twenty-seven bottles were classified as false negative, seven of these had a positive cohort bottle (Staphylococcus aureus, Enterobacter cloacae, Enterococcus faecalis, Candida albicans, Burkholderia cepacia and two strains of Pseudomonas aeruginosa), four out of these seven were not expected to grow in anaerobic media, five patients were under antibiotic treatment and one bottle had a transport time >48 h and is also mentioned in this group. Fifteen of the false negative bottles had a transport time of >48 h. Eleven patients underwent antibiotic therapy, six out of 27 false negative bottles had a transport time <48 h, two patients had antibiotic therapy. Out of these six isolates, one C. glabrata did not grow in the anaerobic bottle.

The rate of clinically relevant false negative blood cultures (pathogen not detected in cohort bottle) in bottles that were entered into BACTEC 9000 within 48 h after inoculation is 0,15%. All efforts need to be directed towards expediting the transportation of material to the microbiology laboratory.

LaboratoriumsMedizin, Walter de Gruyter

Print ISSN: 0025-8466
Volume: 29, 04/2005
Pages: 130 - 135

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