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Jörg Mühling, Marie E. Campos, Armin Sablotzki, Matthias Krüll, Marius G. Dehne, Jens Gonther, Stefan Weiss, Markus Fuchs, Gunter Hempelmann

Effects of Propofol and Taurine on Intracellular Free Amino Acid Profiles and Immune Function Markers in Neutrophils In Vitro

We have examined the effects of propofol, taurine, and the combination of propofol and taurine on amino acid profiles and the immune function markers superoxide anion (O₂^?), hydrogen peroxide (H₂O₂), and released myeloperoxidase (MPO) activity in neutrophils (PMN). Propofol led to significant changes in the dynamic PMN-free amino acid pool. Exogenous taurine significantly reduced PMN neutral amino acid and ?-aminobutyrate (?-aba) as intracellular taurine increased. Incubation with propofol plus taurine resulted in lower intracellular taurine levels and elevated ?-aba and neutral amino acid concentrations compared to propofol alone. Concerning PMN immune function markers, propofol significantly decreased O₂^?-and H formation and released MPO. Taurine led to an increased release of MPO and concomitant significantly reduced O₂^? and H₂O₂ levels. When propofol and taurine were applied together they appeared to act additively with regard to superoxide and hydrogen peroxide formation. In the case of MPO, taurine neutralized propofol's effects, supporting the idea that MPO activity may be regulated by taurine. We believe therefore that taurine is important for strengthening PMN host defense capability, although the mechanisms are not yet clear. Moreover, taurine appears to act primarily by altering the PMN osmotic balance, while propofol seems to affect PMN amino acid metabolism and/or uptake and release.

Clinical Chemical Laboratory Medicine, Walter de Gruyter

Print ISSN: 1434-6621
Volume: 40, 03/2002
Pages: 111 - 121

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