Judith Burri, Max Haldimann
Direct determination of selenium in human serum by inductively coupled plasma-collision cell-mass spectrometry
Background: Selenium status in humans depends on nutritional intake. To facilitate a future monitoring study with a large number of samples, an inductively coupled plasma mass spectrometry (ICP-MS) analysis method including collision cell technology was developed for the direct determination of selenium in human serum.
Methods: Serum samples were diluted (1:15 v/v) with an aqueous solution containing 2-propanol, EDTA(NH4)2, ammonia and Triton X-100. Isotope dilution was used as the calibration method, evaluating the 78Se/82Se ratio. Interference by 81BrH+ was corrected mathematically. Nebuliser blockage was prevented by sample centrifugation and filtration of the wash solution. Method validation was carried out using certified and spiked human sera.
Results: All values obtained for the accuracy tests were either within the certified ranges or showed recovery between 100.9% and 104.7%. Further results were relative standard deviation of 2.0% for repeatability and 3.0% for between-run precision, a limit of detection of 0.5 ?g/L and a limit of quantification of 1.1 ?g/L.
Conclusions: The benefits of the new method are high accuracy combined with low sample consumption and fast sample throughput.
Clin Chem Lab Med 2007;45:895–8.
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 45, 07/2007
Pages: 895 - 898
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