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Dietlinde Büttner, Johannes Büttner

Determination of pH in Human Erythrocytes. Sources of Systematic Error

The results of a simultaneous determination of the pH value in erythrocytes with the potentiometric measuring method and the ¹⁴C-labelled 5,5-dimethyl-2,4-oxazolidinedione (DMO) method showed a mean method difference of 0.026 pH units. The cause of this discrepancy was assumed to be matrix-inherent liquid-junction potentials in the potentiometric measurement. Taking these into account in the calculation leads to consistent values for the methods investigated. The DMO method proved to be free of systematic errors. Another indication of this is that its mean ratio of extracellular to intracellular H⁺ ion concentration (H_e⁺/H_i⁺) substantially agreed with the distribution ratios of other freely diffusible ions and their pH dependence.

Clinical Chemical Laboratory Medicine, Walter de Gruyter

Print ISSN: 1434-6621
Volume: 36, 10/1998
Pages: 757 - 761

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