Z.-J. Pang, F.-Q. Xing
Comparative study on the expression of cytokine – receptor genes in normal
and preeclamptic human placentas using DNA microarrays
Aims: To study the relationship between the expression
levels of cytokine/receptor genes in placenta and the
pathogenesis of pre-eclampsia.
Methods: The study was performed to compare the
mRNA contents of cytokine (receptor) superfamily
genes in placentas from 5 patients with pre-eclampsia
and 5 strictly matched normal pregnancies. A complementary
DNA microarray representing over 220 cytokine-associated genes was employed to complete the
detection.
Results: It was shown that, among the 221 kinds of cytokine-associated genes, 162 of those including 22 interleukin/interleukin receptor genes presented with a difference
of over two times in pre-eclamptic placentas
compared to normal placentas. Most of the 22 interleukin/interleukin receptor genes were shown to be
highly expressed in preeclamptic placenta, while the expression
of IL-2 receptor (IL-2R?, GenBank: X01057)
gene in preeclamptic placenta was comparatively lower
than that in normal placenta. Furthermore, some tumor
necrosis factor (TNF)/receptor superfamily genes, including
TNF (GenBank: X02910), TNF ligands (Gen-Bank: U03398, U37518, AF053712, AF055872) and TNF
receptors (GenBank: X60592, X63717, M83554,
AF016266, AF016267, U81232) were also shown to be
highly expressed in pre-eclamptic placenta. Besides interleukin
and tumor necrosis factor (receptor) gene superfamily,
the mRNA levels of another 39 cytokine and
15 cytokine receptor genes showed a two-fold difference
between pre-eclamptic and normal placental tissues.
Additionally, most of the genes were up-regulated in
pre-eclamptic placenta.
Conclusions: The up-regulation of cytokine-associated
genes including interleukin and TNF (receptor) superfamily
expression in placenta might be intensively related
to the pathogenesis of pre-eclampsia.
Journal of Perinatal Medicine, Walter de Gruyter
Print ISSN: 1619-3997
Volume: 31, 03/2003
Pages: 153 - 162
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