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Olaf Rolf, Bernd Baumann, Thomas Sterner, Norbert Schtze, Franz Jakob, Jochen Eulert, Christof Paul Rader
Characterization of mode II – wear particles and cytokine response in a human macrophage – like cell culture / Charakterisierung von Mode II – Abriebpartikeln und der Zytokinantwort in einer humanen Makrophagen – ähnlichen
Keywords: Particle disease, mode II wear, aseptic loosening, cytokine response, Partikelkrankheit, Mode II-Abrieb, aseptische Prothesenlockerung, Zytokinantwort
Informations about wear particles in metallosis (mode II wear) and their effects in vitro and in vivo are limited. The aim of this study was to characterize wear particles obtained intraoperatively and to analyse their effects on cytokine response in an established human macrophage -like cell culture model [15].
Method: Wear particles were obtained intraoperatively from four patients with metallosis resulting from CrCoMo/PE/TiAlV-implants (mode II wear) (3 knee, 1hip prosthesis). After purification, particles were characterized regarding to their composition and size (particle size analyser, electron microscopy, edx-analysis, histological slices). The effects of particles on the release of cytokines (PDGF, IL-1β, IL-8, TNF α) were determined in an established human macrophage-like cell culture system by ELISA-assays.
Results: The metal wear particles consisted of TiAlV with a mean size of 0,1± 0,15 μm, independent of the prosthesis location. CrCoMo particles could not be detected.
In the cell culture model 1456 × 108 particles per 1 × 106 macrophages released maximum amounts of TNFα (8-fold) and IL-8 and IL-1β (5-fold) while the survival rate of the cells was more than 90 percent. A particle-dependent increase of PDGF-levels could not be detected.
Conclusion: As already shown for mode I wear particles (contact between primary bearing surfaces), also mode II wear particles cause release of bone resorbing cytokines in a macrophage-like cell culture model. Because their local and systemic effects in vivo are still not completely understood, we recommend a complete removal of wear particles in cases of metallosis to avoid possible immunological reactions of the body as well as periprosthetic osteolysis.
Biomedizinische Technik, Walter de Gruyter
Print ISSN: 0013-5585
Volume: 50, 02/2005
Pages: 25 - 29