Corrina Powell, Nicolas Orsi, Nigel Simpson, Malcolm Levene
Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood
Objective: Abnormal inflammatory responses are implicated in the pathogenesis of neonatal disease. This study aimed to describe the neonatal cytokine response using an in vitro model of stimulated cord blood.
Methods: Cord blood samples (n = 12) were incubated in RPMI 1640 medium with and without lipopolysaccharide. Concentrations of tumor necrosis factor (TNF)-?, interleukin (IL)-6, IL-8, interferon (IFN)-? and IL-10 were determined by multiplex immunoassay at 0, 1, 3, 6 and 24 hours of incubation. The difference between stimulated and control response was defined as the potential secretory capacity (mean ± S.E.M.; pg/million white cells). Analysis included a Kruskal-Wallis test and post-hoc Mann-Whitney U test.
Results: All cytokine capacities increased rapidly by 1 hour (p < 0.001), except IL-10 (p = 0.04). TNF-? peaked between 3–6 hours (1581 ± 377 pg/million WC), declining by 24 hours. Similarly, IFN-? peaked at 3 hours. Capacity ascended throughout the incubation period for IL-6, IL-8 (631 ± 75 pg/million WC) and IL-10 (311 ± 37 pg/million WC). Overall, IFN-? capacity was lowest (72 ± 10 pg/million WC) and IL-6 capacity was greatest (61489 ± 7059 pg/million WC).
Conclusion: The neonatal inflammatory response is chronologically similar to that determined in adults. Immature neonatal T-cell function may account for the lower IFN-? production. These results may expand our knowledge of neonatal disease, etiology and management.
Journal of Perinatal Medicine, Walter de Gruyter
Print ISSN: 1619-3997
Volume: 32, 09/2004
Pages: 440 - 445
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