M. Kim, G. Kim, R. Romero, S.-S. Shim, E.-C. Kim, B. H. Yoon
Biovar diversity of Ureaplasma urealyticum in amniotic fluid: distribution,
intrauterine inflammatory response and pregnancy outcomes
Objective: The objective of this study was to determine
the distribution of two biovars of Ureaplasma urealyticum
(parvo and T960) in human amniotic fluid and
to examine whether the magnitude of the intrauterine
inflammatory response and pregnancy outcomes are different
between patients with microbial invasion of the
amniotic cavity with “parvo biovar” and those with
“T960 biovar”.
Study design: This cohort included 77 preterm singleton
pregnancies (gestational age < 37 weeks) in whom U. urealyticum was detected from amniotic fluid using the
polymerase chain reaction (PCR). Amniotic fluid was
obtained by transabdominal amniocentesis. Amniotic
fluid was cultured for aerobic and anaerobic bacteria as
well as mycoplasmas. U. urealyticum was biotyped by
PCR methods. Amniotic fluid inflammatory response
was determined by amniotic fluid white blood cell count
and interleukin-6 concentration.
Results: 1) The “parvo biovar” was detected in 82%
(63/77) and “T960 biovar” was in 18% (14/77) of cases; 2)
U. urealyticum was isolated by conventional culture
method from amniotic fluid in 56% (35/63) of cases with
positive for “parvo biovar” and in 50% (7/14) of cases
with positive for “T960 biovar”; 3) There were no significant
differences in the median gestational age at amniocentesis,
gestational age at delivery, birth weight, amniotic
fluid white blood cell count, amniotic fluid interleukin-6
concentration and the rates of clinical chorioamnionitis,
histologic chorioamnionitis, funisitis and neonatal morbidity
between patients in the two biovar groups.
Conclusions: 1) The “parvo biovar” is more frequently
isolated from amniotic fluid of preterm gestations than
the “T960 biovar”; 2) Biovar diversity of U. urealyticum
in amniotic fluid was not associated with different pregnancy
outcome and magnitude of the intraamniotic inflammatory
response.
Journal of Perinatal Medicine, Walter de Gruyter
Print ISSN: 1619-3997
Volume: 31, 03/2003
Pages: 146 - 152
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