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Zahur Zaman, Sandy De Spiegeleer, Mathieu Gerits, Norbert Blanckaert
Analytical and Clinical Performance of Two Cardiac Troponin I Immunoassays
Cardiac troponin I assays for Axsym (Abbott Diagnostics, Abbott Park, IL, USA) and Immuno 1 (Bayer Corporation, Tarrytown, NY, USA) analysers were evaluated. Heparin plasma or serum could be used for both assays. Samples were stable for 24 h at ambient temperature, 3 days at 4–8 ? and 3 months at ?20 °C. After 10 months' storage at ?80 °C, the recoveries were well above 100 % by both assays. Total coefficients of variation for Axsym assay were 9.0 %, 5.8 % and 5.3 % at concentrations of 2.6 ?g/l, 9.83 ?g/l and 34.3 ?g/l respectively; for Immuno 1 these were 4.4 %, 1.6 % and 1.8 % at 2.3 ?g/l,6.27 ?g/l and 44.35 ?g/l respectively. It was ? 20 % at concentration of ? 0.5 ?g/l for Axysm assay and ? 0.15 ?g/l for Immuno 1 assay. Recoveries were ? 90 % at ? 0.22 ?g/l on Axsym and at ? 1.47 ?g/l on Immuno 1. Neither method showed significant interference with haemoglobin, bilirubin, triglycerides or rheumatoid factor. Correlation between the two methods was excellent (r = 0.997, Y (Axsym) = 4.2X (Immuno 1) + 3.2). The highest concentrations detected in 50 healthy subjects were 0.3 ?g/l and 0.1 ?g/l by Axsym and Immuno 1 methods, respectively. Twelve out of 43 renal failure patients had troponin I 0.13–0.9 ?g/l using Axsym method and 4 had levels of 0.07–0.13 ?g/l using Immuno 1. In muscle trauma patients, troponin I was undetectable.
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 37, 09/1999
Pages: 889 - 897