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Renata Zadro, Mirna Su?i?, Igor Aurer, Jasminka Metelko-Kova?evi?, Boris Labar, Ana Stavljeni? Rukavina
Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR
Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations.
Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products.
By splitting the multiplex primer mix, the patient specific T-cell receptor ? rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor ? gene rearrangement. Three patients exhibited two rearranged T-cell receptor ? genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments.
Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 36, 08/1998
Pages: 637 - 639