S. J. Fortunato, R. Menon, N. U. Ahmed, M. Bourgeois, G. A. Dildy
Amniotic fluid concentrations of collagenase-1 and collagenase-3 are increased in polyhydramnios
Objective: Polyhydramnios places increased tension on
the fetal membranes and should conceptually be associated
with an increased need for tissue remodeling to
prevent premature rupture of membranes (PROM). Herein
we use polyhydramnios as a model to study the association
of the collagenase class of matrix metalloproteinases
(MMPs) with tissue remodeling during pregnancy.
Methods: Amniotic fluids were collected by transabdominal
amniocentesis from women with polyhydramnios and
from women with normal amniotic fluid volume at second
trimester, third trimester and at term. Fluids were assayed
for MMP-1 and MMP-13 (collagenase-1 and 3) using
ELISA. The concentrations in polyhydramnios were compared
with subjects undergoing amniocentesis with normal
amniotic fluid volume. Statistical comparisons were
made using Scheffe's method for correction in multiple
means comparison. P of <0.05 was considered statistically
significant.
Results: MMP-1 and MMP-13 are present in the amniotic
fluid at all stages of pregnancy. The concentrations
of these MMPs were significantly elevated in the polyhydramnios
samples independent of gestational age.
Conclusion: Polyhydramnios is associated with
increased amniotic fluid concentrations of MMP-1 and
MMP-13 suggesting their role in tissue remodeling.
Journal of Perinatal Medicine, Walter de Gruyter
Print ISSN: 1619-3997
Volume: 32, 03/2004
Pages: 122 - 125
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