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Sophie Séronie-Vivien, Florence Dalenc, Patrick Balaguer, Jean-Claude Nicolas, Henri Roché, Jean-Charles Faye
A recombinant cell bioassay for measurement of overall estrogenic activity of serum: preliminary results in women with breast cancer
Keywords: breast cancer, estrogenic activity, MELN cells, recombinant bioassay, serum
The estrogenic status of patients with breast cancer may influence the prognosis and the response to treatment and is currently assessed by immunological measurement of serum estradiol. This does not account for estrogenic or anti-estrogenic activity related to growth factors able to activate the estrogen receptor, to anti-estrogenic drugs or to exogenous supply of estrogen-like compounds. We developed a recombinant bioassay based on a mammary cell line expressing luciferase in an estrogen receptor-dependent way. In a human serum matrix the MELN system was able to detect the transcriptional activity of estradiol, growth factors (epidermal growth factor (EGF), insulin at insulin-like growth factor 1 (IGF-1)-like concentrations), xeno-estrogens (diethylstilbestrol, phytoestrogens) and tamoxifen in a dose-dependent manner. The intra- and inter-assay variations were <6% and ?15%, respectively, whatever the estradiol concentration; the functional sensitivity was <10 pmol/l equivalents of estradiol. We assessed the overall estrogenic activity of serum (OEAS) in 16 healthy women and in 24 women with advanced breast cancer. The correlation between OEAS and serum log1017-?–estradiol (E2) was good for healthy women (r2 = 0.8568) but poor for patients (r2 = 0.0563). Assessment of the OEAS/E2 ratio as a prognostic and predictive factor would be of interest in clinical prospective trials involving ER+ breast cancer patients.
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 42, 08/2004
Pages: 889 - 895