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E. Sabrinah Chapman, Martin Sorette, Edward Hetherington, David Zelmanovic, Gail Kling, Jacques Dugailliez, Nuria Pujol-Moix, David Okrongly
A rapid, automated flow cytometric method to measure activated degranulated platelets by density determination
Platelet activation is reported to correlate with acute coronary syndromes. A platelet analysis method on the ADVIA 120 Hematology System provides rapid analysis of platelet density, reported as mean platelet component (MPC) concentration, utilizes routine hematology specimens, requires no pre-treatment, and thirty seconds to generate results. Sub-populations of platelets separated by density gradients showed excellent correlation with the ADVIA 120 MPC parameter (r = 0.997). Platelet activation induced by thrombin treatment resulted in a shift of platelets into the lowest density fraction (d1.068 g/mL) with a corresponding reduction in MPC from 24.7 to 20.6 g/dL, N = 4 subjects (p < 0.004). There was also excellent correlation between expression of CD62P measured by fluorescence flow cytometry and the ADVIA 120 Hematology System MPC values (r = 0.85). These results indicate that the ADVIA 120 MPC parameter may be a useful new test for assessing activated platelets and platelet density.
Thrombosis and Haemostasis, Schattauer
Print ISSN: 0340-6245
Volume: 89, 06/2003
Pages: 1004 - 1015