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Diran Herebian, Uta Kpper, Dietmar Schomburg, Franz-Josef Marner

In vivo labeling with stable isotopes as tool for the identification of unidentified peaks in the metabolome analysis of Corynebacterium glutamicum by GC/MS

Keywords: Corynebacterium glutamicum, GC/MS, in vivo labeling, metabolic profiling, metabolite identification, Stable isotopes

Of the hyphenated techniques, used for metabolic profiling of cell and tissue extracts, GC/MS is in some ways advantageous, as it allows the simultaneous fingerprinting of chemically very different metabolites and the recorded EI mass spectra lead in many cases to the unambiguous identification of the compounds. However, prior to chromatography, the hydrophilic substances of the cell extracts have to be converted to vaporizable derivatives, the mass spectra of which often are not known or not listed in the available spectral libraries, even when they are derived from simple biochemicals. Thus, numerous chromatographic peaks remain as yet unidentified. Attempts to identify these peaks afford the acquisition of more data on the compounds. The value of in vivo labeling of the metabolites with ¹³C and ¹⁵N for this purpose is described.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 2007
Pages: -

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