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Marco Tlio R. Gomes, Marcelo P. Bemquerer, Miriam Tereza P. Lopes, Michael Richardson, Sergio Oyama Jnior, Carlos E. Salas

The structure of CMS2MS2, a mitogenic protein isolated from Carica candamarcensis

Keywords: Caricaceae, cysteine proteinase, latex, mitogenic

In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cells proliferation. Our purpose here is to determine the primary structure of CMS2MS2 and predict its tridimensional structure. The protein contains 214 residues including the catalytic tryad composed by Cys₂₅, His₁₅₉, Asn₁₇₅. A phylogenetic tree analysis demonstrated that CMS2MS2 ranks closer to chymopapain than to papain. The overall predicted three-dimensional structure is similar to proteases from the papain family. These results suggest that minor structural differences within CMS2MS2 must account for its proliferative action.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 2007
Pages: -

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