M. Malanga, B. Farina
Noncovalent Binding of Poly(ADP-Ribose) to Nuclear Matrix Proteins: Developmental Changes and Tissue Specificity
Poly(ADP-ribose) is a nuclear polynucleotide involved
in the regulation of chromatin functions via covalent
and/or noncovalent modification of nuclear proteins.
Using a binding assay on protein blots, we searched
for poly(ADP-ribose) binding proteins in nuclear matrices
from testes of differently aged rats as well as from
various adult rat tissues (brain, liver, spleen). We found
that nuclear matrix proteins represent a significant
subset of the nuclear proteins that can establish noncovalent
interactions with poly(ADP-ribose). The profiles
of poly(ADP-ribose) binding nuclear matrix proteins
appeared to be tissue-specific and changed
during postnatal development in the testis.
The isolation and analysis of endogenous poly
(ADP-ribose) from rat testes showed that the ADP-ribose
polymers that bind nuclear matrix proteins in
vitro are also present under physiologic conditions in
vivo. These results further substantiate the possibility
that poly(ADP-ribose) may affect chromatin functions
through noncovalent interaction with specific protein
targets, including nuclear matrix components.
Biological Chemistry, Walter de Gruyter
Print ISSN: 1431-6730
Volume: 381, 11/2000
Pages: 1047 - 1053
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