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G. Dorff, G. Meyer, D. Krone, P. Pozzilli, H. Zühlke
Neuronal NO Synthase and Its Inhibitor PIN Are Present and Influenced by Glucose in the Human ?-Cell Line CM and in Rat INS-1 Cells
Nitric oxide (NO) is synthesised by different nitric oxide synthases (NOS) from Larginine and acts as a signal transducer in a variety of cells. The neuronal isoenzyme of NOS (nNOS) was recently found in rodent ?cells and ?cell lines. We provide evidence that nNOS is also present in the human ?cell line CM and that the specific inhibitor of nNOS PIN is expressed in CM and INS-1 cells. Furthermore, we investigated the influence of glucose on the activity of nNOS and the expression of PIN and are able to show that both are increased by glucose stimulation in the ?cell lines but not in the mouse fibroblastic cell line LTK. This indicates that nNOS and PIN play a role in the specific function of ?cells, not only in rodents but also in humans.
Biological Chemistry, Walter de Gruyter
Print ISSN: 1431-6730
Volume: 383, 09/2002
Pages: 1357 - 1361