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Joachim M. Weitzel, Stefan Grott, Christiane Radtke, Sabine Kutz, Hans J. Seitz

Multiple Promoters Direct the Tissue-Specific Expression of Rat Mitochondrial Glycerol-3-Phosphate Dehydrogenase

The mitochondrial FADdependent glycerol-3-phosphate dehydrogenase (mGPDH) is an essential component of the glycerol phosphate shuttle which transfers reduction equivalents from the cytoplasm into the mitochondria. We analyzed the distribution of different exon 1-containing transcripts by RTPCR in various tissues in vivo. Exon 1a was predominantly expressed in brain, brown adipose tissue and pancreas, exon 1b was ubiquitously expressed, and exon 1c was exclusively expressed in testis. In transient transfection assays the ubiquitous promoter B showed a detectable activity, whereas promoters A and C were completely silent. A deletion mutational analysis located the basal promoter B activity to a 316 bp core sequence upstream of the transcription start site.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 381, 08/2000
Pages: 611 - 614

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