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Guillaume Morissette, François Marceau

Molecular identification and pharmacological profile of the bovine kinin B₁ receptor

Keywords: bovine genome, Endothelial cells, kinin B1 receptor, pharmacological antagonism

To support the study of kinin pharmacology in bovine models of cultured endothelial cells (ECs), the Bovine Genome Project was searched for a B₁ receptor (B₁R) gene ortholog (BDKRB1). A contig complementary to an intronless coding nucleotide sequence was found. The sequence was amplified from bovine EC DNA, further cloned into pcDNA3 and expressed in COS-1 cells. The bovine B₁R sequence was confirmed and extended. A putative Zn²⁺-binding motif HEXXH is not present (replaced by HDAWP). The receptor binds [³H]Lys-des-Arg⁹-bradykinin in a saturable manner (K_d 0.36 nM) and exhibits a pharmacological profile similar to that of human B₁R.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 387, 02/2006
Pages: 211 - 215

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