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Ulrike Stadtmüller, Jana Eben-Brunnen, Angela Schmid, Dörte Hesse, Simone Klebert, Hartmut D. Kratzin, Jan Hesse, Bodo Zimmermann, Susanne Reymann, Friedrich P. Thinnes, Roland Benz, Hilde Götz, Norbert Hilschmann

Mitochondria-Derived and Extra-Mitochondrial Human Type-1 Porin Are Identical as Revealed by Amino Acid Sequencing and Electrophysiological Characterisation

In mammalian cells porin channels are localised in both mitochondrial outer membranes and extra-mitochondrial membranes. We isolated mitochondria-derived porin of a human lymphoblastoid B cell line, determined its amino acid sequence and characterised its channel properties. Interestingly, the amino acid sequence of this porin preparation and, correspondingly, its electrophysiological characteristics in a reconstituted system were identical to those of ‘Porin 31HL’, the human type-1 porin purified from a crude membrane preparation of the same cell line using a different purification protocol. The results raise questions about targeting, insertion and orientation of human type-1 porin in different membranes.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 380, 12/1999
Pages: 1461 - 1466

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