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Toshihide Kobayashi, Konstantin Startchev, Andrew J. Whitney, Jean Gruenberg

Localization of Lysobisphosphatidic Acid-Rich Membrane Domains in Late Endosomes

Late endosomes accumulate internal membranes within the lumen of the organelle. These internal membranes are enriched in the late endosome specific phospholipid, lysobisphosphatidic acid (LBPA). The organization of LBPArich membrane domains is not well characterized. Using an LBPAspecific monoclonal antibody (6C4), we show that these membrane domains are not accessible from the cytoplasm. Using fluorescence correlation spectroscopy, we also show that 6C4 only binds sonicated, but not intact, late endosomes, presumably reflecting the release of internal membranes upon endosome rupture.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 382, 03/2001
Pages: 483 - 485

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