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Hirokazu Hara, Mitsuhiro Ohta, Kiyoe Ohta, Masataka Nishimura, Hiroshi Obayashi, Tetsuo Adachi

Isolation of Two Novel Alternative Splicing Variants of Allograft Inflammatory Factor-1

Allograft inflammatory factor-1 (AIF-1) was initially cloned from rat cardiac allografts undergoing chronic rejection. AIF-1, possessing an EF-hand-like motif, is thought to be a Ca²⁺ binding protein. In this study, we identified two novel alternatively spliced variants of AIF-1 by reverse-transcription polymerase chain reaction. One variant encodes an AIF-1 protein that lacks 14 amino acids corresponding to one exon. The other variant encodes a truncated AIF-1 protein due to a frameshift introduced by an 85-bp insertion, and its C-terminal region differs from that of AIF-1. Interestingly, these variants have incomplete and no EF-hand-like motifs, respectively. The expression level of the latter variant is high in peripheral blood leukocytes, and it is selectively expressed in macrophage-like cell lines.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 380, 11/1999
Pages: 1333 - 1336

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