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Angelika Bondzio, Anita Abraham-Podgornik, Petra Blankenstein, Siegfried Risse

Involvement of Intracellular Ca2+in the Regulation of Bovine Leukemia Virus Expression

The calcium ionophore A23187, which was used to i n c rease the intracellular calcium concentration ([Ca[2+]]), was analyzed for effects on bovine leukemia virus (BLV) expression in two BLV infected cell lines. To clarify the role of intracellular free calcium in this response, [Ca[2+]] was measured during ionophore treatment with the fluorescent calcium indicator Fura-2. Elevation of intracellular calcium under these conditions caused an enhancement of BLV gp51 and p24 synthesis as well as an activation of the BLV long terminal repeat (LTR) in a dosedependent manner. Furthermore, it was observed that elevated levels of intracellular calcium following A23187 stimulation lead to activation of NF?B . Based on inhibitor studies, we hypothesize that the effect of A23187 on BLV expression appears to be mediated by PKC.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 382, 03/2001
Pages: 407 - 416

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