You are here: Home :: Area NEM :: Life sciences :: Biochemistry

M.S. El-Halawany, S. Ohkouchi, H. Shibata, K. Hitomi, M. Maki

Identification of cysteine protease inhibitors that belong to cystatin family 1 in the cellular slime mold Dictyostelium discoideum

Family 1 cystatins are cytosolic inhibitors of cysteine proteases, and they are conserved in higher eukaryotes. We characterized two newly identified family 1 cystatins of the cellular slime mold Dictyostelium discoideum, cystatin A1 and A2. Their recombinant proteins showed specific inhibitory activity against papain and cathepsin B, respectively. Using specific polyclonal antibodies, we found that cystatin A1 is stably expressed throughout the life cycle of Dictyostelium, whereas cystatin A2 expression is up-regulated during the course of development.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 385, 06/2004
Pages: 547 - 550

Show full article (external site)

Show all available items of this journal

 

Area NEM