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Masaki Hayama, Yuushi Okumura, Etsuhisa Takahashi, Aki Shimabukuro, Manabu Tamura, Noriaki Takeda, Takeshi Kubo, Hiroshi Kido
Identification and analysis of the promoter region of the type II transmembrane serine protease polyserase-1 and its transcript variants
Keywords: E-box, gene regulation, polyserase-1, promoter, serase-1B, serase-2B, type II transmembrane serine proteases
Polyserase-1/TMPRSS9 and its alternative transcripts, serase-1B and serase-2B, are novel type II transmembrane serine proteases that may regulate physiological and pathological phenomena on the cell surface. To understand mechanisms of these transcripts gene expression and regulation, we cloned and characterized the 5' promoter region of the mouse polyserase-1 (mpolyserase-1) gene. Using 5'-rapid amplification of cDNA ends analysis, we located the transcription initiation site 272 nucleotides upstream of the translation initiation site. Luciferase reporter gene analysis revealed that the region from +186 to +272 bp in the 5'-untranslated region (UTR), containing the GATA motif (AGATAA), glucocorticoid responsible element (TGTTCT), and E-box sequence (CAGGTG), was required for maximal promoter activity. Mutations introduced into the E-box sequence but not elsewhere in the promoter region caused selective reduction of transcriptional activity. Furthermore, a DNA probe (+229 to +255 bp) containing the E-box sequence formed a single nuclear protein complex in a sequence-specific manner. These data suggest that the expression of mpolyserase-1 and its transcript variants are positively regulated by the E-box in its 5'-UTR that might be responsible for basic helix-loop-helix transcription factors involved in the development of various organelle.
Biological Chemistry, Walter de Gruyter
Print ISSN: 1431-6730
Volume: 2007
Pages: -