Detlev Mennerich, Sonja Hoffmann, Thorsten Hadrys, Hans-Henning Arnold, Eva Bober
Two Highly Related Homeodomain Proteins, Nkx5-1 and
Nkx5-2, Display Different DNA Binding Specificities
The mouse Nkx5-1 and Nkx5-2 genes are related to NK
genes in Drosophila and encode proteins with very
similar homeodomains. In higher vertebrates Nkx5
genes are specifically expressed in the inner ear. Inactivation
of the mouse Nkx5-1 gene by homologous recombination
revealed a critical role for the formation
of vestibular inner ear structures. Here, we investigated
biochemical properties of the proteins encoded
by the Nkx5 genes. A similar consensus binding sequence
was isolated for both Nkx5 proteins using
binding site selection. This sequence is related to target
sequences previously identified for other Nkx proteins
and contains the conserved homeodomain binding
core TAAT. An additional, novel and unrelated high
affinity binding sequence could be identified for the
Nkx5-2 protein.
Biological Chemistry, Walter de Gruyter
Print ISSN: 1431-6730
Volume: 380, 09/1999
Pages: 1041 - 1048
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