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Gabriella Fibbi, Silvia DAlessio, Marco Pucci, Massimiliano Cerletti, Mario Del Rosso
Growth Factor-Dependent Proliferation and Invasion of Muscle Satellite Cells Require the Cell-Associated Fibrinolytic System
The process of muscle regeneration in normal and dystrophic muscle depends on locally produced cytokines and growth factors and requires the activity of the urokinase plasminogen activator/urokinase plasminogen activator receptor/plasminogen activator inhibitor-1 system. In this study we tested the effect of basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF) and transforming growth factor? (TGF?) on the fibrinolytic pattern of normal and dystrophic satellite cells, their mitogenic and motogenic activities and the dependence of such activities on the cellassociated fibrinolytic system. We have observed that the urokinase plasminogen activator (uPA) receptor is weakly upregulated by bFGF in normal satellite cells, while it is strongly upregulated by TGF?, mainly in dystrophic myoblasts. bFGF upregulated uPA in both normal and dystrophic myoblasts grown in primary culture, while a striking downregulation was observed with TGF?. TGF? was the only growth factor able to exceptionally upregulate plasminogen activator inhibitor-1 (PAI-1), mainly in dystrophic satellite cells. HGF did not show any activity on the fibrinolytic system. Proliferation and invasion into Matrigel matrices of normal and dystrophic cells occurred regardless of the growth factordependent regulation of the fibrinolytic system. Nevertheless, each growth factor required the efficiency of the constitutive cellassociated fibrinolytic system to operate, as shown by impairment of growth factor activity with antagonists of uPA and of its receptor. Noteworthy, TGF? induced a dosedependent increase of Matrigel invasion only in dystrophic myoblasts. Since TGF?challenged dystrophic myoblasts undergo an exceptional upregulation of the receptor and of PAI-1, we propose the possibility that the TGF?induced fibrinolytic pattern (low urokinase plasminogen activator, high receptor and high PAI-1) may be exploited to promote survival and spreading of transplanted engineered myoblasts in Duchenne muscular dystrophy.
Biological Chemistry, Walter de Gruyter
Print ISSN: 1431-6730
Volume: 383, 01/2002
Pages: 127 - 136