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Vathany Kulasingam, Eleftherios P. Diamandis

Glucocorticoid receptor-mediated expression of kallikrein 10 in human breast cancer cell lines

Keywords: betamethasone, cell culture, dexamethasone, differential regulation, Mifepristone, steroid hormones

Using the breast cell lines MCF-10A, MDA-MB-468 and T-47D, we investigated the role of various glucocorticoids in regulating human kallikrein 10 (KLK10) expression. We found that increased concentrations of glucocorticoids decreased KLK10 expression in MCF-10A cells and increased KLK10 expression in MDA-MB-468 and T-47D cells. Stimulation of the cell lines using other steroid hormones did not induce any difference in KLK10 expression in MCF-10A and MDA-MB-468 cells, suggesting that regulation of KLK10 occurs primarily through glucocorticoids. However, T-47D cells expressed higher levels of KLK10 upon dihydrotestosterone stimulation. Blocking the glucocorticoid receptor (GR) demonstrated that the mechanisms of induction and repression are different in the three cell lines studied. Taken together, our results propose an alternative mode of KLK10 regulation, namely by glucocorticoids via GR-dependent mechanisms.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 2007
Pages: -

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