You are here: Home :: Area NEM :: Life sciences :: Biochemistry

M. Siewi?ski, Y. Saleh, A. Popiela, P. Ziólkowski, M. Jele?, M. Grybos

Expression of High Molecular Weight Cysteine Proteinase Inhibitor in Ovarian Cancer Tissues: Regulation of Cathepsin B Expression by Placental CPI

Mouse polyclonal antibodies against placental cysteine proteinase inhibitor (CPI) react with the placental 67 kDa CPI on Western blots, and CPI present in ovarian cancer homogenate and serum was shown by double immunodiffusion to react with the same antiserum. By immunohistochemical staining, positive expression of high molecular weight CPI was observed on the tumour cell surface in serous and endometrioid ovarian carcinomas with metastasis. Normal endometrioid tissue was not stained with anti-placental CPI antibodies. Cathepsin B and procathepsin B median levels in ovarian cancer tissue homogenates increased progressively with FIGO stage of the disease. The enzyme level decreased 22-fold after treatment of tissue homogenates with 5 nM purified CPI. These results provide evidence that addition of CPI reduces the levels of cysteinetype cathepsins to those of normal non-cancerous values.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 384, 07/2003
Pages: 1103 - 1107

Show full article (external site)

Show all available items of this journal

 

Area NEM