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Francisco Boán, Miguel G. Blanco, Paula Barros, Jaime Gómez-Márquez

DNA end-joining driven by microhomologies catalyzed by nuclear extracts

Keywords: double-strand break, microhomologies, minisatellite MsH42, non-homologous end-joining, recombination

In a previous work we used an in vitro system for the generation and analysis of double-strand breaks (DSBs) using nuclear extracts from rat testes as a source of DSB activity. Since the recombination process can be triggered by the formation of DSB, in the present study we developed a strategy to isolate and characterize recombinant molecules using the same in vitro system. Our results indicate that the mechanism for the formation of recombinants was non-homologous end-joining driven by microhomologies. The procedure described here represents an alternative to investigate the mechanisms of DNA end-joining and other forms of DNA repair.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 387, 03/2006
Pages: 263 - 267

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