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C. Haslin, M. Pellegrini

Culture Medium Composition for Optimal Thallus Regeneration in the Red Alga Asparagopsis armata Harvey (Rhodophyta, Bonnemaisoniaceae)

The formation and the growth of regenerated fronds from cultivated barbs of Asparagopsis armata were investigated according to an experimental design methodology. A maximal ratio of 3.3 mm regeneration/mm barb was reached for two media constituted of seawater complemented with iron sulfate, EDTA and potassium bromide. The first optimal medium was composed of [FeSO₄7H₂O] = 0.20 mg L^?1, [Na₂EDTA2H₂O] = 2.9 mg L^?1, [KBr] = 12.0 mg L^?1 and the second one of [FeSO₄7H₂O] = 0.2 mg L^?1, [Na₂EDTA2H₂O] = 4.6 mg L^?1, [KBr] = 28.7 mg L^?1. Barbs excised from adult thalli were placed for 4 weeks in seawater enriched with several chemical substances at different concentrations. The experimental procedure involved: 1) the screening of the influencing compounds with an Hadamard design and 2) the investigation of their optimal concentrations according to a Doehlert design of response surface methodology.

Botanica Marina, Walter de Gruyter

Print ISSN: 0006-8055
Volume: 44, 01/2001
Pages: 23 - 30

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