Elide A. Pastorello, Claudio Ortolani, Chiara Baroglio, Valerio Pravettoni, Marco Ispano, Maria Gabriella Giuffrida, Donatella Fortunato, Laura Farioli, Mara Monza, Lorenzo Napolitano, Marco Sacco, Elisabetta Scibola, Amedeo Conti
Complete Amino Acid Sequence Determination of the
Major Allergen of Peach (Prunus persica) Pru p1
The major protein allergen of peach (Prunus persica),
Pru p1, has recently been identified as a lipid transfer
protein (LTP). The complete primary structure of
Pru p1, obtained by direct amino acid sequence and
liquid chromatography-mass spectrometry (LC-MS)
analyses with the purified protein, is described here.
The protein consists of 91 amino acids with a calculated
molecular mass of 9178 Da. The amino acid sequence
contains eight strictly conserved cysteines, as
do all known LTPs, but secondary structure predictions
failed to classify the peach 9 kDa protein as an
‘all-alpha type’, due to the high frequency of amino
acids (nine prolines) disrupting alpha helices. Although
the sequence similarity with maize LTP is only
63%, out of the 25 amino acids forming the inner surface
of the tunnel-like hydrophobic cavity in maize
ns-LTP 16 are identical and 7 similar in the peach homolog,
supporting the hypothesis of a similar function.
Biological Chemistry, Walter de Gruyter
Print ISSN: 1431-6730
Volume: 380, 11/1999
Pages: 1315 - 1320
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