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Cristinel Sandu, Peter Nick, Daniel Hess, Emil Schiltz, Timothy A. Garrow, Roderich Brandsch
Association of Betaine-Homocysteine S-Methyltransferase with Microtubules
In mammals, betaine of the mitochondrial matrix is used in the cytosol by betainehomocysteine Smethyltransferase for methionine synthesis. The resulting dimethylglycine is shuttled back into the mitochondrial matrix for further degradation. Nanospray tandem mass spectrometry and Nterminal amino acid sequencing of microtubuleassociated proteins from rat liver tubulin revealed that betainehomocysteine Smethyltransferase is microtubule associated. This was confirmed by confocal laser scanning microscopy of HepG2 cells labeled with betainehomocysteine Smethyltransferase and ?tubulinspecific monoclonal antibodies. The association of betainehomocysteine Smethyltransferase with the cytoskeleton may functionally integrate the mitochondrial and cytoplasmic compartments of choline degradation.
Biological Chemistry, Walter de Gruyter
Print ISSN: 1431-6730
Volume: 381, 08/2000
Pages: 619 - 622