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Enrico Grazi, Orietta Cintio, Ermes Magri, Giorgio Trombetta

Anomalous Binding of MgADP to Myosin of Skeletal Muscle

Binding of adenosine diphosphate to skeletal muscle myosin was studied using a range of concentrations from 0 to 2mM. Up to 0.2mM adenosine diphosphate two equivalent and independent nucleotide binding sites were detected, characterized by the single association constant of 5 × 10⁴M^?1. At greater adenosine diphosphate concentrations a decreasing binding capacity was noticed, bound nucleotide being essentially ~ 0.1 mol/mol at a 1–2mM adenosine diphosphate concentration. We tentatively propose that nucleotides act indirectly on myosin by promoting the perturbation of the solvent, which is supported by the fact that polyphosphates are known powerful kosmotropes.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 381, 01/2000
Pages: 35 - 38

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