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P. K. Grover

A Simple Single-Step Method for the Synthesis of Recombinant Non-Homologous Competitor cDNA and Its Implications for Quantitative PCR in Biological Sciences and Molecular Diagnostics

In recent years there has been great interest in quantitative polymerase chain reaction. Consequently, a large number of assays have been developed, of which the one using nonhomologous competitors is arguably the most precise. Despite widespread applications, currently there is no simple method to synthesize such competitors. Here a facile and costeffective, singlestep method for synthesis of recombinant, nonhomologous, competitor complementary DNA is described. The method can be adapted to generate competitors of any size and sequence. The entire procedure is quick, straightforward and does not require any specialized equipment except a standard thermocycler.

Biological Chemistry, Walter de Gruyter

Print ISSN: 1431-6730
Volume: 383, 09/2002
Pages: 1463 - 1469

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