P. O. Hassa, M. O. Hottiger
A Role of Poly (ADP-Ribose) Polymerase in NF- B
Transcriptional Activation
The transcription factor NF-?B plays a critical role in immune and inflammatory responses. Here we show that poly (ADP ribose) polymerase (PARP) is required for specific NF-?B transcriptional activation in vivo. The activation of the HIV-LTR promoter and an NF-?Bdependent artificial promoter was drastically reduced in PARP (_/_) cells, independently of the signaling pathway through which NF-bB was induced. Furthermore NF-?B-dependent gene activation was restored in vivo by the expression of PARP in PARP (_/_) cells. Finally, we show that both NF-?B and PARP formed a stable immunoprecipitable nuclear complex. This interaction did not need DNA binding. Our results suggest that PARP is an important cofactor in the activation cascade of NF-?B-dependent target genes.
Biological Chemistry, Walter de Gruyter
Print ISSN: 1431-6730
Volume: 380, 07/1999
Pages: 953 - 959
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