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A. R. Jalilian, A. Khorrami, M. B. Tavakoli, M. Kamali-Dehghan, Yari Y. Kamrani

Development of [²⁰¹Tl](III)-DTPA-human polyclonal antibody complex for inflammation detection

Keywords: 201Tl(III)-complex, Biodistribution, Human polyclonal antibody, inflammation

Thallium-201 (T_1/2=3.04 d) in Tl⁺ form was converted to Tl³⁺ cation in presence of O₃ in 6 M HCl controlled by RTLC/gel electrophoresis methods and used in the labeling of human polyclonal antibody (HIgG) after conjugation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the addition of 1 mL of a HIgG pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=7) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25 °C with continuous mild stirring for 30 min. The final isotonic [ ²⁰¹Tl](III)-DTPA-HIgG complex was checked by radio-TLC using several solvent systems to ensure the formation of only one species followed by filtration through a 0.22 μ filter (specific activity= 33.7 TBq/mM, radiochemical purity >95%). Preliminary bio-distribution studies in normal and inflammation-bearing rats were performed. The target/skin and target/blood ratios were 4 and 6 after 28 h respectively, showing the selectivity of the radiopharmaceutical for the inflammatory lesions.

Radiochimica Acta, Oldenbourg Wissenschaftsverlag

Print ISSN: 0033-8230
Volume: 95, 11/2007
Pages: 669 - 675

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