You are here: Home :: Area NEM :: Agricultural science

Martin Kruså, Gunnar Henriksson, Gunnar Johansson, Torbjörn Reitberger, Helena Lennholm

Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium: A model compound study

Keywords: cellobiose dehydrogenase, cellulose biodegradation, Hydroxyl radical, methyl ?-D-glucopyranoside

Cellobiose dehydrogenase (CDH) is an extracellular enzyme produced by various wood-degrading fungi. It oxidizes cellobiose to cellobionolactone under reduction of molecular oxygen to hydrogen peroxide, and Fe³⁺ to Fe²⁺. These activated agents can thereafter form highly reactive hydroxyl radicals, which depolymerize wood polymers. In this work, cellulose depolymerization by CDH was studied using a model compound, methyl ?-D-glucopyranoside. The formation of glucose, arabinose, gluconic acid, erythrulose and formaldehyde were detected and a mechanism for the reaction is proposed. The biological importance of this enzyme-initiated reaction is discussed.

Holzforschung, Walter de Gruyter

Print ISSN: 0018-3830
Volume: 59, 05/2005
Pages: 263 - 268

Show full article (external site)

Show all available items of this journal

 

Area NEM