M. Lawoko, A. Nutt, H. Henriksson, G. Gellerstedt, G. Henriksson
Hemicellulase Activity of Aerobic Fungal Cellulases
Summary
Cellulases isolated from Trichoderma reesei and Phanerochaete chrysosporium were screened for hemicellulolytic,
pectinolytic and cellulolytic activity using locust bean mannan, birchwood xylan, citrus fruit
pectin and carboxymethylated cellulose (CMC) as substrates. The purpose of this work was to choose
appropriate enzymes to include in a “miniature cellulase system” with minimal hemicellulase activity
for the preparation of lignin-carbohydrate complexes (LCCs). The endoglucanases showed CMC activity
whereas activity towards the substrate was not detected for the CBHs. Xylanase activity was observed
for EG I and EG 38 whereas mannanase activity was observed for EG 44. None of the enzymes degraded
pectin. The results suggest that CBH I, CBH II, CBH 58, EG II and EG III are good candidates for
the effective preparation of LCCs. The possible biological function for the hemicellulolytic activity of
cellulases is discussed.
Holzforschung, Walter de Gruyter
Print ISSN: 0018-3830
Volume: 54, 09/2000
Pages: 497 - 500
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