Y. Hosoo, M. Yoshida, T. Imai, T. Okuyama
Diurnal Differences in the Innermost Surface of the S2 Layer in Differentiating Tracheids of Cryptomeria japonica Corresponding to a Light-Dark Cycle
Summary
This paper describes the effect of light on the diurnal change in the innermost surface of developing
secondary walls. Cryptomeria japonica D. Don saplings were grown in two growth chambers, in
which temperature and relative humidity were kept constant and the light-dark phase of the photoperiod
varied. One chamber reproduced the natural light-dark phase, while the other reversed it.
Samples of differentiating xylem were collected during the dark period when the tangential strain,
used as an index of volumetric changes in differentiating cells, was high, and during the light period
when the tangential strain was low. The innermost surface of developing secondary walls in differentiating
tracheids was observed by field emission scanning electron microscopy. In the specimens
collected during the dark period, amorphous material was observed and the cell wall surface was
immunogold-labeled with an anti-glucomannan antiserum. In the specimens collected during the
light period, cellulose microfibrils were clearly evident, and amorphous material and immunogold
labeling were rarely observed. These results demonstrate that the diurnal changes in the innermost
surface of developing secondary walls correspond to the light-dark cycle over 24 h.
Holzforschung, Walter de Gruyter
Print ISSN: 0018-3830
Volume: 57, 10/2003
Pages: 567 - 573
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