You are here: Home :: Area NEM :: Agricultural science

M. Ragnar, T. Eriksson, T. Reitberger

Detection of Radicals Generated by Strong Oxidants in Acidic Media

A new method to study superoxide and hydroxyl radicals in aqueous solution in the pH range 1 to 6 is described and critically evaluated. The method primarily detects superoxide, which reacts with tetranitromethane (TNM) by reductive cleavage. The strongly coloured nitroform anion (?_350nm = 15000M^?1 cm^?1) formed, is easily measured in a spectrophotometer or in a stopped-flow equipment. Hydroxyl radicals are measured indirectly. In a first step the hydroxyl radical reacts with iso-propyl alcohol to give acetone and superoxide. The superoxide formed is then in a second step detected with TNM. The TNM-method was primarily designed to monitor the radical formation under ozone bleaching conditions, but it is also applicable in acidic hydrogen peroxide.

Holzforschung, Walter de Gruyter

Print ISSN: 0018-3830
Volume: 53, 05/1999
Pages: 285 - 291

Show full article (external site)

Show all available items of this journal

 

Area NEM