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Andrzej Leonowicz, Jerzy Rogalski, Magdalena Jaszek, Jolanta Luterek, Maria Wojtas-Wasilewska, Elzbieta Malarczyk, Grazyna Ginalska, Monika Fink-Boots, Nam-Seok Cho
Cooperation of Fungal Laccase and Glucose 1-Oxidase in Transformation of Björkman Lignin and Some Phenolic Compounds
A screening of wood-rotting basidiomycete fungi was conducted for glucose 1-oxidase (GOD) and laccase (LAC) production as well as for ligninolytic activity measured by a Rhemazol reaction. The results showed that genera rich in GOD are lignin degraders as well as effective producers of extracellular LAC. The fungi poor in GOD neither showed LAC, nor ligninolytic activity. The Björkman lignin and 3 phenolic compounds, hydroquinone and syringic and vanillic acids, were tested on the sequential activity of LAC and GOD. In the presence of LAC, quinoid intermediates formed from Björkman lignin and phenolic compounds were observed. The addition of GOD caused a diminution of the quinone level. During incubation of Björkman lignin with LAC and GOD depolymerization occurred, and in the experiments omitting GOD the quantities of low molecular products were markedly lower. Consequently, the consecutive ping-pong activity of LAC and GOD reduced the polymerization and improved the efficiency of depolymerization processes.
Holzforschung, Walter de Gruyter
Print ISSN: 0018-3830
Volume: 53, 07/1999
Pages: 376 - 380