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Heng Gao, Todd F. Shupe, Chung Y. Hse, Thomas L. Eberhardt

Antioxidant activity of extracts from the bark of Chamaecyparis lawsoniana (A. Murray) Parl.

Keywords: antioxidant activity, Folin-Ciocalteu method, free radical scavenger, methanol extracts, Port-Orford cedar, total phenolic content

The bark of Chamaecyparis lawsoniana (A. Murray) Parl. was extracted with methanol and sequentially partitioned with n-hexane, ethyl acetate, n-butanol and deionized water. The antioxidant activities of the four extracts were evaluated using the DPPH? and ABTS+? methods. The total phenolic content of the extracts was determined using the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). Butylated hydroxytoluene was used as a positive control in the radical-scavenging activity tests. All the bark extracts showed significant radical-scavenging activity. In the ABTS+? assay, n-butanol extracts exhibited the strongest radical-scavenging activity, followed by ethyl acetate, water, and n-hexane extracts. The greatest total phenolic content was 428.54 mg GAE per gram of dry extract and was detected in the n-butanol extract, followed by the ethyl acetate and n-hexane extracts. The antioxidant activities correlate with the amount of phenolics present in these extracts. The ethyl acetate and n-butanol extracts were rich in phenolics and may represent a good source of antioxidants.

Holzforschung, Walter de Gruyter

Print ISSN: 0018-3830
Volume: 60, 07/2006
Pages: 459 - 462

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